 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 2]](ba0051fig2mag.jpg)
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Figure 2
The principle of 3D reconstruction from 2D projections. A set of four different molecular orientations, displayed as rendered iso-surfaces, are shown above their corresponding 2D projections. Real cryo-EM images are very noisy versions of these projections. The Fourier transform of each projection, shown below the projections, is a section through the 3D Fourier transform of the structure (DeRosier & Klug, 1968  ). The 3D transform is represented by two intersecting transform sections, derived from the side and end views of the structure. Once enough sections are available, the full 3D transform can be interpolated and inverse transformed into a 3D density map. The map is shown with the backbone of the structure to emphasize the fact that the full 3D density is obtained, including internal features. Surface rendering is generally used to represent such maps when the resolution is insufficient to provide an atomic model of the structure. |
![[Figure 2]](ba0051fig2.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
