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Figure 4
Negative-stain and cryo-EM. A virus particle on a carbon support film is shown in schematic side view. The particle is outlined with good contrast by heavy-metal stain, but is somewhat flattened by the accompanying dehydration. It is preserved in the native hydrated state in the cryo-sample but the protein–ice contrast is very low. In order to maximize the contrast, the cryo-specimen is imaged over holes in the carbon support film.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 56| Part 10| October 2000| Pages 1215-1222
doi:10.1107/S0907444900010787
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