Post-translational modification of the N-terminal His tag interferes with the crystallization of the wild-type and mutant SH3 domains from chicken src tyrosine kinase

Kim, K.M.; Yi, E.C.; Baker, D.; Zhang, K.Y.J.

doi:10.1107/S0907444901002918

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 1
Analysis of recombinant src SH3 by electrophoresis. (a) Silver-stained SDS–PAGE of wild-type SH3 treated with thrombin (lane 1), SH3 after removal of thrombin and His-tagged fragments (lane 2) and SH3 from affinity chromatography and before thrombin treatment (lane 3). (b) Silver-stained IEF electrophoresis analyses of recombinant SH3 fused with and without His tag are shown in lanes 1 and 2, respectively.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 57| Part 5| April 2001| Pages 759-762

doi:10.1107/S0907444901002918

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