Crystallization and preliminary X-ray diffraction studies of glycerol 3-phosphate cytidylyltransferase from Staphylococcus aureus

Yim, V.C.N.; Zolli, M.; Badurina, D.S.; Rossi, L.; Brown, E.D.; Berghuis, A.M.

doi:10.1107/S0907444901005212

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 1
Crystals of TarD_Sa obtained during optimization of growth conditions. (a) Crystals of TarD_Sa grown in the presence of CTP and MgCl₂ obtained from a sparse-matrix screen. (b) Crystals obtained after refining precipitant and protein concentrations. (c) Results of adding D(+) glucose to the crystallization conditions. (d) Final optimized crystals of TarD_Sa obtained by reducing the rate of vapour diffusion. Photographs were taken under polarized light.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 57| Part 6| May 2001| Pages 918-920

doi:10.1107/S0907444901005212

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