Figure 2
(a) Stereographic projection showing the ordinary self RF of human malic enzyme. Reflection data between 10 and 3.5 Å resolution were used in the calculation with the program GLRF (Tong & Rossmann, 1990). The radius of integration was 35 Å. (b) Stereographic projection showing the orientation of the NCS twofold axes based on the top peak (red symbols) and the next three peaks (green, purple and cyan symbols, respectively) in the locked self RF. The top peak is the correct solution and the next three peaks arise from accidental overlap with one of the twofold axes of the NCS. |