Figure 2
κ = 180° sections of self-rotation functions showing the directions of the twofold axes of symmetry. All were calculated using POLARRFN (Collaborative Computational Project, Number 4, 1994). (a) Using data generated from the EM image of hepatitis B showing the viral T = 4 symmetry. Resolution range 50–10 Å and search sphere radius 60 Å. (b) Using X-ray data for HepB. The map is viewed down the crystallographic twofold axis. Resolution range 20–8 Å and search sphere radius 60 Å. (c) Using X-ray data for gp6. Crystal symmetry requires there be 13 twofold axes. Resolution range 30–12 Å and search sphere radius 50 Å. (d) Using X-ray data for gp6. Resolution range 80–12 Å. The 13 twofold axes have been obscured by the pattern generated from the solvent content of the crystal. |