Figure 3
The 〈F〉 distributions plotted against resolution for the observed X-ray data and the calculated structure factors generated from the EM images. (a) The distribution for gp6 X-ray data in the range 80–8.2 Å. The distribution is quite smooth, but increases rapidly at very low resolution. (b) The distribution for X-ray data from HepB in the range 30–7 Å. (c) The distribution of 〈F〉 obtained from the gp6 EM images. This was normalized then `blunted' by the application of an artificial temperature factor of 400 Å2 before being used as model data for molecular-replacement searches. The information from 12–8.2 Å did not improve the molecular-replacement signal. (d) The distribution of 〈F〉 obtained from the HepB EM images. These images showed more detail than those for gp6 and the distribution from 20 to 7.4 Å fell off at much the same rate as that of the X-ray data. Including them in the MR calculations also improved the signal. |