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Figure 4
(a) Any line perpendicular to the tangent of a circle passes through the centre of a circle. For the roughly circular object shown in (b), lines in the direction of the steepest gradient do not cross at a single point, but the crossing points can be used to estimate the centre and provide a measure of circularity.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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