Figure 7
The SAD phase ambiguity. If the positions of anomalous scatterers are known, their scattering contribution can be calculated. (a) With the accurately measured amplitudes of two Friedel mates, FT+ and , there are two possible solutions for the total protein phase φT placed symmetrically around φA − 90°, the direction of the anomalous vector . (b) If the measurement errors of the Friedel amplitudes are taken into account, the protein phase can be described by a predictable probability distribution. |