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Figure 9
The second derivative in the isomorphous replacement (a) or the second-wavelength data in MAD (b) indicates which alternative solution is correct. In MAD it is, however, necessary that the [f'] contribution has to be different at the two wavelengths; otherwise, the selection cannot be achieved, even if [f''] values are different. Therefore, in the MAD approach the [f''] values indicate the two possible values of the protein phase and the dispersive differences in [f'] provide an indication which of the two alternatives is correct.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 58| Part 11| October 2002| Pages 1958-1967
doi:10.1107/S0907444902016645
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