 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 1]](za5022fig1mag.jpg)
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Figure 1
Coomassie-stained 12% SDS gel of different pools during purification of KFD→ERC. (a) Lane M, molecular-weight markers (kDa); lane 1, crude lysate (soluble protein band seen at 30 kDa); lane 2, lysate after interaction with Ni–NTA agarose beads (the 30 kDa protein has bound to the column and hence is not seen in the lane); lane 3, purified KFD→ERC after metal-affinity purification. (b) Lane 1, affinity-purified KFD→ERC after desalting into the anion-exchange buffer; lane 2, purified (>95%) KFD→ERC after gel filtration. |
![[Figure 1]](za5022fig1.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
