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Figure 2
Coomassie-blue stained SDS–PAGE (12%) and tritium FdUMP-labelling film showing expression and purification of the PfDHFR-TS. Lane M, low-molecular-weight protein markers as indicated; lanes 1 and 2, purified PfDHFR-TS samples from MTX and Q-Sepharose columns, respectively; lanes 3 and 4, Coomassie blue staining and autoradiograph of Q-purified PfDHFR-TS with tritium FdUMP labelling and tritium signal enhancer embedding, respectively; lanes 5 and 6, dissolved crystals of PfDHFR-TS–WR99210–NADPH–dUMP complex; lanes 7 and 8, tritium FdUMP-labelling films of lanes 5 and 6, respectively.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 60| Part 4| April 2004| Pages 780-783
https://doi.org/10.1107/S0907444904001544
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