Figure 1
A silver-stained 10–20% gradient SDS–PAGE gel showing the progress of purification of tomatinase. Lane M, molecular-weight markers (kDa); lane 1, culture filtrate (100 ng total protein); lane 2, pooled fractions after POROS HQ anion-exchange chromatography (10 ng total protein); lane 3, purified tomatinase (10 ng protein) |