Figure 1
Location of HR regions used in this study in the F-protein gene and their amino-acid sequences. (a) Schematic diagram of SeV F protein showing F1 and F2 and the functional/structural modules (domains) in addition to HR1 and HR2. SS, signal sequence; FP, fusion peptide; IFP, internal fusion peptide; LZ, leucine-zipper domain (amino acids 201–229); TM, transmembrane region. `S—S' represents the disulfide bond linking F1 and F2. (b) Sequence alignment of HR1 and HR2 predicted by LearnCoil-VMF (Singh et al., 1999) or used by Shai and coworkers and here (our construct is named 2-Helix, in which HR1 and HR2 are linked by a linker, SGGRGG.) |