Cloning, expression, purification, crystallization and preliminary X-ray characterization of the full-length single-stranded DNA-binding protein from the hyperthermophilic bacterium Aquifex aeolicus

Clarke, D.J.; Northey, C.G.; Mack, L.A.; McNae, I.W.; Alexeev, D.; Sawyer, L.; Campopiano, D.J.

doi:10.1107/S0907444904020530

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 2
Analysis of the crystallized DNA-bound SSB protein by ESI–MS. The main figure shows the deconvoluted mass of 17 127.3 (obtained using Transform software; Micromass UK), consistent with the theoretical value of 17 131.2; right inset, ion envelope of crystallized SSB protein; left inset, SDS–PAGE analysis of crystallized SSB protein results in a single band running at an anomalous weight of ∼23 kDa.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 60| Part 11| November 2004| Pages 2009-2012

https://doi.org/10.1107/S0907444904020530

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