Figure 1
Schematic representation of coronavirus MHV A59 spike protein and the MHV 2-Helix constructs. S1 and S2 are formed after proteolytic cleavage (vertical arrow) and noncovalently linked. The enveloped protein has an N-terminal signal sequence (SS) and a transmembrane domain (TM) adjacent to the C-terminus. S2 contains two HR (heptad-repeat) regions (hatched bars), termed HR1 and HR2. FP (hatched bars) is a putative fusion peptide followed by an HR1 region. Two HR regions were linked to a single polypeptide with an eight-residue linker (GGSGGSGG). |