Figure 1
12% SDS–PAGE showing the purification of RacE. Lane 1, Mark12 MW protein markers (kDa) (Invitrogen); lane 2, crude extract; lane 3, combined fractions after DEAE anion-exchange chromatography; lane 4, combined fractions after hydrophobic chromatography; lane 5, RacE protein collected after gel-filtration chromatography. |