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Figure 1
Comparison of electron-density maps in molecular-replacement phasing for crystals with 60% solvent. All maps were calculated using the coordinates of M. jannaschii IF5A (PDB code 2eif ; Berman et al., 2000BB4; Kim et al., 1998BB10) and measured amplitudes from crystals of P. aerophilum IF5A (PDB code 1bkb ; Peat et al., 1998BB15). Coordinates of M. jannaschii IF5A are in gray (including the labeled residue Tyr119) and those of P. aerophilum IF5A are in black (including the labeled residue Tyr79). (a) σA-weighted (Read, 1986BB18) (2mFobsDFcalc)exp(iφcalc) map. (b) Prime-and-switch map. (c) Omit map. (d) Statistical density-modification-based (Terwilliger, 2000BB22) map. Contours are at 1.0σ in the omit map and 1.5σ in all others.

Journal logoBIOLOGICAL
ISSN: 1399-0047
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