Exploitation of non-crystallography symmetry (NCS) coupled to side-chain docking in data collected from a syntenin crystal. Frags. 1, 2 and 3 are three fragments produced by the main-chain tracing algorithm (a), while Mol1 and Mol2 are produced by NCS-based extension (b). Successful side-chain docking allows the construction of a sequence alignment versus the protein monomer (template) sequence (c) from which NCS operators can be calculated. These NCS operators can be used to build a template molecule which, when mapped back onto the fragments, leads to a more complete model in three-dimensional space (b) as well as in sequence space (d). Asterisks delimit the start and/or end of the extensions, except for the N- and C-termini.