Figure 1
SDS–polyacrylamide gel analysis at different stages during purification. Positions of molecular-weight standards are indicated on the left and the four subunits of complex II are labeled on the right. Lane 1, whole mitochondria. Lane 2, residue from detergent extraction. Lane 3, detergent extract. Lane 4, pooled fractions, TSK column. Lane 5, pooled flowthrough fractions, hydroxyapatite column. Lane 6, pooled fractions from DEAE-Sepharose elution. Lane 7, ammonium sulfate fractionation, 40% saturation pellet. Lane 8, pooled fractions from Sephacryl S-300 column. Lane 9, pooled fractions from glycerol density-gradient centrifugation. |