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Figure 4
Optimization procedures using three rows of the 96-well plate. (a) Basic procedure varying the pH, reservoir concentration and protein/reservoir ratio, (b) specific optimization for SARS coronavirus nsp9 protein (OPPF 406). The original crystals were obtained from block 2, solution E9 [2 M (NH4)2SO4, 0.1 M citrate–phosphate pH 4.2]. The structure was determined using the crystals from wells F3, G2 and H3 (Sutton et al., 2004BB17). The scale bar represents 200 µm.

Journal logoBIOLOGICAL
ISSN: 1399-0047
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