A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization

Walter, T.S.; Diprose, J.M.; Mayo, C.J.; Siebold, C.; Pickford, M.G.; Carter, L.; Sutton, G.C.; Berrow, N.S.; Brown, J.; Berry, I.M.; Stewart-Jones, G.B.E.; Grimes, J.M.; Stammers, D.K.; Esnouf, R.M.; Jones, E.Y.; Owens, R.J.; Stuart, D.I.; Harlos, K.

doi:10.1107/S0907444905007808

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 4
Optimization procedures using three rows of the 96-well plate. (a) Basic procedure varying the pH, reservoir concentration and protein/reservoir ratio, (b) specific optimization for SARS coronavirus nsp9 protein (OPPF 406). The original crystals were obtained from block 2, solution E9 [2 M (NH₄)₂SO₄, 0.1 M citrate–phosphate pH 4.2]. The structure was determined using the crystals from wells F3, G2 and H3 (Sutton et al., 2004

). The scale bar represents 200 µm.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 61| Part 6| May 2005| Pages 651-657

doi:10.1107/S0907444905007808

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