 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
journal menu
![[Figure 5]](ic5029fig5mag.jpg)
|
|
Figure 5
(a) Crystals after the first round of an additive screen on protein target L1. The initial hit in 2.0 M ammonium sulfate, 0.1 M Na acetate pH 4.6 gave an overnucleated drop where one could distinguish microcrystals (not shown). Addition of 4% poly propylene glycol 400, leads to cubic crystals to form. (b) Iteration of the additive kit with a solution containing 2.0 M ammonium sulfate, 0.1 M Na acetate pH 4.6, 4% PPG400. Larger crystals are formed in the drop containing 3% ethylene glycol. Glycerol could also be used as an additive with the same effect and was also used as the cryoprotectant. (c) Needle clusters of Target 228 obtained in the initial crystal screen 16% PEG 4000, 0.1 M MgCl2, 0.1 M Tris pH 8.5. (d) Condition from salt screen, where 0.1 M MgCl2 was replaced by 0.1 M KCl: thin needles are produced. (e) Screening of additives using condition (d) showed that 0.1 M guanidine improved notably crystal size. |
![[Figure 5]](ic5029fig5.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
