 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 1]](dz5036fig1mag.jpg)
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Figure 1
(a) A schematic illustration of the novel mounting technique. (i) Picking up a protein crystal using a nylon loop at the top of glass micropipette and mounting the tools on the goniometer head intercepting the cryostream. (ii) Aspirating the cryo-buffer through the micropipette and flushing with the cold nitrogen stream. (iii) Removing the nylon loop under cryo-conditions. (b) The mounting base for the novel mounting technique. This tool can hold the micropipette and aspirate the cryo-buffer through the flexible tube. (c) Left, the tip of the micropipette and nylon loop before picking up a protein crystal. The nylon loop was glued to the tip of the micropipette. Middle, the tip of the micropipette and a protein crystal after aspirating the cryo-buffer and freezing. Right, the tip of the micropipette and a protein crystal after removing the nylon loop. In the middle and right pictures, a thaumatin (Sigma T-7638) crystal was used as the sample. |
![[Figure 1]](dz5036fig1.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
