Figure 4
The consequences of radiation damage (wild-type complex). (a) A σA-weighted difference map shows negative density (red) at the disulfide bridge and positive density (green) consistent with an extra atom attached to Cβ of Cys7 in trypsin; (b) the interpretation as a partially occupied disulfide bridge and a partially occupied serine instead of Cys7. The contouring levels for the density maps were 1σ (σA map) and 3σ for the difference density map. |