 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 2]](ba5085fig2mag.jpg)
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Figure 2
Optimization of cryobuffer. Diffraction patterns illustrating the effect of adding increasing concentrations of glycerol to water. For 100% water, discrete reflections from individual ice crystals are observed. As the concentration of glycerol is raised, the diffraction shows typical sharp powder pattern rings from the ice crystallites, becoming a single sharp ring and then a diffuse ring with gentle gradients on both the high- and low-resolution sides. Adequate cryoprotection has been achieved with 45%:55%(v/v) glycerol:water. The resolutions at which ice reflections appear are also tabulated. |
![[Figure 2]](ba5085fig2.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
