 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 5]](ba5085fig5mag.jpg)
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Figure 5
Examples of different icing problems. (a) Ice on, within and around sample. Loop too far from end of cryostat nozzle (5–8 mm recommended) and not correctly aligned in the cold nitrogen stream. After correction of alignment and distance, annealing could be tried and might rescue this sample. (b) Flow-rate of outside dry air/nitrogen stream not matched to inner cold nitrogen stream, so that turbulence on the gas interface draws in wet warm air from the room. Moisture from the air drops out giving a cylindrical wall of frost round the sample. Adjustment of the dry air/nitrogen flow will clear this and for this sample, a data set was subsequently successfully collected. (c) An ice blob deposited on a crystal during storage or transport. This can be cleared by brushing the sample with a small artist's brush or pouring a small volume of liquid nitrogen over the loop (taking care not to crack the beamline camera lens if it is directly below the sample). |
![[Figure 5]](ba5085fig5.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
