 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 8]](ba5085fig8mag.jpg)
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Figure 8
Visual changes to samples owing to X-ray irradiation. (a) A needle crystal (approx 20 × 20 × 300 µm) of complement component protein Factor I that has been exposed to a 100 × 100 µm2 beam at ID14-3, ESRF, Grenoble for a total of 45 min. The cryobuffer was 25% PEG600 and 0.1 M imidazole malate pH 4.5. (Roversi et al., 2004  ). (This photograph was kindly provided by Pietro Roversi.) (b) Crystal of Salmonella typhimurium neuraminidase cryocooled to 16 K using a helium gas stream at ID14-4, ESRF, Grenoble after a few seconds of X-ray exposure. The crystal was in phosphate buffer (2 M K2HPO4:0.43 M KH2PO4) with 40% glycerol as the cryoprotectant agent. The buffer alone is responsible for the colour change. (c) A crystal of bacteriorhodopsin exposed at 100 K for ten images at each of 13 places to a 30 µm diameter X-ray beam on ID19 at the ESRF, Grenoble. (d) After the exposures, the crystal was allowed to warm up to room temperature, and it disintegrated, releasing gas which can be seen as bubbles. The black scale bar represents 50 µm. (Photographs c and d were kindly provided by Tassos Perrakis.) |
![[Figure 8]](ba5085fig8.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
