Figure 9
B-value increase and specific structural damage inflicted on a cryocooled crystal of apoferritin during sequential data sets collected at ID14-4, ESRF: (a) and (b) structure coloured from blue (20 Å2) to red (100 Å2) by crystallographic B-value for (a) data set 1 and (b) data set 10 [plotted using Molscript (Kraulis, 1991) and Raster3d (Merritt & Bacon, 1997)], (c) 2Fo − Fc map of Glu63 contoured at 0.2 e Å−3 after data set 1 and (d) after data set 10. (e) 2Fo − Fc map of Met96 contoured at 0.2 e Å−3 after data set 1 and (f) after data set 10, showing specific structural damage [maps are from Coot (Emsley & Cowtan, 2004), and plotted using Raster3d]. |