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Figure 1
Crystals of sSEMA4D. (a) Hexagonal needles obtained with 0.1 M MES pH 6.0, 1 M LiCl and 20%(w/v) PEG 6000. (b) Thin rectangular plates obtained with 0.1 M HEPES pH 7.0, 1 M LiCl and 20%(w/v) PEG 6000. (c) Thicker rectangular tiles obtained with 0.1 M Tris–HCl pH 8.0, 1 M LiCl and 20%(w/v) PEG 6000. (d) A crystal obtained from 0.2 M Na2SO4 and 20%(w/v) PEG 3350 after 3 d. (e) The same crystal after 5 d. (f) Crystal of SeMet sSEMA4D obtained in 0.2 M NH4F and 20%(w/v) PEG 3350 by seeding with native sSEMA4D microcrystals obtained under the same conditions.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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