 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 3]](ba5086fig3mag.jpg)
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Figure 3
Phasing of sSEMA4D data using MAD. (a) Selenium sites obtained from the PK-COMB combined peak data set demonstrating the expected twofold non-crystallographic symmetry (shown by the horizontal line; the pair of sites toward the left-hand edge are the low-occupancy sites). (b) Electron-density map (contoured at 1σ) for the phased PK-COMB data set after density modification and cross-crystal form averaging against NAT (spheres mark Se-atom sites). (c) Electron-density map (contoured at 1.2σ) obtained from the MAD-phased PK1 data set after phase improvement with RESOLVE showing the basic β-propeller structure (yellow lines show a roughly superposed Cα trace for the αVβ3 integrin β-propeller). (d) Sample of 2Fobs − Fcalc electron density (blue lines, contour level 1σ) and Fobs − Fcalc difference density (red lines, contour level −3σ; green lines, contour level +3σ) obtained using the HIRES data set and the final refined model showing radiation damage to the disulfide linkage between residues B260 and B305. Figure prepared using BOBSCRIPT (Esnouf, 1997  , 1999) and rendered with RASTER3D (Merritt & Murphy, 1994). |
![[Figure 3]](ba5086fig3.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
