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Figure 2
DNA truncation and mutagenesis methods. (a) Generation of specific constructs by PCR, (b) unidirectional DNA truncation using exonuclease III and nuclease (mung bean or S1), (c) fragmentation using enzymatic or physical breakage of the DNA, (d) random point mutation by error-prone PCR (epPCR) or bacterial mutator strain and (e) DNA shuffling for combining different lineages of point mutations on the same DNA strand.

Journal logoBIOLOGICAL
ISSN: 1399-0047
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