view article

Figure 4
Overview of different methods of assaying for protein solubility. Low-throughput studies typically involve fractionation of expression lysates by centrifugation and SDS–PAGE. Increased throughput can be achieved by fractionating lysates using a filter plate and analysis of fractions by dot blot using an antibody against a fused epitope. For larger libraries, cell-based screens or selections employing reporter fusions can be used in which the solubility of the protein is coupled to the phenotype of the fusion partner. Approximate numbers of clones that can be measured are indicated.

Journal logoBIOLOGICAL
ISSN: 1399-0047
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds