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Figure 3
Process of the large-scale protein crystallization and monitoring system PXS. (a) Crystallization plate supplier. (b) Dispenser 1. Deep-well plates containing precipitant solution are placed on the turntable. The dispensing head with 96 disposable tips is seen in the centre. (c) Dispenser 2. The first dispensing head with one disposable tip dispenses protein solution from one Eppendorf tube to eight wells on a middle plate seen on the right. (d) The second dispensing head of dispenser 2 with eight disposable tips. (e) Dispenser 3 with 96 disposable tips aspirates precipitant solutions from 96 wells and mixes them with protein drops on the crystallization plate. (f) The sealer. (g) Carrying robot and observation system. The carrying robot with two arms improves the total throughput of the system. (h) Inside of the incubator. There is an automatic double door for importing and exporting crystallization plates at the opposite side of the emergency door, which is open to give a clear view of the inside. (i) Close-up view of the observation system. The crystallization plate is illuminated from underneath and an image of each drop is acquired by the colour CCD camera above the plate. The plate is fixed on the stage and both the light source and the camera move synchronously to take images of the 96 wells.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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