Figure 1
Influence of controlled dehydration on (a) the unit-cell parameters and (b) mosaic spread and 〈I/σ(I)〉 of a crystal of bovine F1-ATPase. The crystal orientation was fixed and still diffraction patterns were recorded every 5 min, corresponding to a decrease in relative humidity of 0.5%. (a) The changes in the a and c unit-cell parameters with relative humidity are plotted as solid and dotted lines, respectively. The relative humidity is plotted as filled circles. The b unit-cell parameter decreases by less than 1 Å during dehydration. (b) The change in the mosaic spread and 〈I/σ(I)〉 for the outer shell (4.75–4.44 Å) are plotted as solid and dotted lines, respectively. The relative humidity is plotted as filled circles. |