Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE

Alzari, P.M.; Berglund, H.; Berrow, N.S.; Blagova, E.; Busso, D.; Cambillau, C.; Campanacci, V.; Christodoulou, E.; Eiler, S.; Fogg, M.J.; Folkers, G.; Geerlof, A.; Hart, D.; Haouz, A.; Herman, M.D.; Macieira, S.; Nordlund, P.; Perrakis, A.; Quevillon-Cheruel, S.; Tarandeau, F.; van Tilbeurgh, H.; Unger, T.; Luna-Vargas, M.P.A.; Velarde, M.; Willmanns, M.; Owens, R.J.

doi:10.1107/S0907444906029775

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 1
Schematic representation of the LIC-PCR protocol. The procedure for cloning into the LIC-PCR vector pET-YSBLIC (Table 2

) is outlined. In (a) the method for preparing the vector is described and in (b) the preparation of the PCR fragment and annealing to the vector is shown.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 62| Part 10| September 2006| Pages 1103-1113

doi:10.1107/S0907444906029775

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