 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 3]](gx5081fig3mag.jpg)
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Figure 3
Expression of the protein W00005 in either pETG-10A (5′ att recombinatorial site included in the translation product) or pDEST14 (5′ att recombinatorial site excluded from the translation product). A parallel expression experiment was performed in E. coli BL21(DE3) using the two Gateway-compatible vectors, pETG-10A (Table 2 ![[link]](../../../../../../logos/arrows/d_arr.gif) ) and pDEST14 (InVitrogen). The bacterial cultures were grown at 310 K to OD of 0.6 at 600 nm and induced with 50 µM IPTG at 303 K for 4 h. Equal amount of cells (based on the OD at 600 nm) were withdrawn for solubility analysis. Cells were lyzed by sonication and soluble (lane S) and insoluble fractions (lane P) were separated by centrifugation. Proteins were captured from the supernatant fraction using Ni–NTA agarose beads (Qiagen) (lane E). |
![[Figure 3]](gx5081fig3.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
