 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 4]](gx5081fig4mag.jpg)
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Figure 4
Colony blot of 27 000 clones, arrayed in duplicate, expressing a randomly truncated target gene (code OPTIC 1612). Each clone from the deletion library is printed as an inoculum onto a nitrocellulose filter and grown at 310 K to give the colony array. Expression is induced by transferring the colony array onto LB agar containing IPTG. Following lysis in situ, the array is screened for constructs that express soluble proteins. These are detected using a linear peptide fused to the constructs that is efficiently post-translationally modified in vivo only if the protein is both soluble and stable (manuscript in preparation). |
![[Figure 4]](gx5081fig4.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
