Figure 1
Analysis by MS of a nitrogen regulatory protein from N. meningitides before and after crystallization. (a) Mass spectrum of the purified protein prior to crystallization. The preparation contained both the phosphorylated and the non-phosphorylated protein form. (b) Image of the crystal taken for MS analysis. The protein was crystallized in 100 mM citrate pH 5 and 3.2 M ammonium sulfate. (c) Mass spectrum of the crystallized protein sample prepared using the protocol described in Nettleship et al. (2005). The crystal only contained the non-phosphorylated protein form. |