The impact of protein characterization in structural proteomics

Geerlof, A.; Brown, J.; Coutard, B.; Egloff, M.-P.; Enguita, F.J.; Fogg, M.J.; Gilbert, R.J.C.; Groves, M.R.; Haouz, A.; Nettleship, J.E.; Nordlund, P.; Owens, R.J.; Ruff, M.; Sainsbury, S.; Svergun, D.I.; Wilmanns, M.

doi:10.1107/S0907444906030307

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 2
Thermal shift assay results for a protein measured at constant ionic strength in different buffers with varying pH values. The melting temperature (T_m) and therefore the stability of the protein decreases with increasing pH. Below pH 6 the protein precipitated completely and no fluorescence signal was recorded.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 62| Part 10| September 2006| Pages 1125-1136

doi:10.1107/S0907444906030307

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