 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 3]](gx5084fig3mag.jpg)
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Figure 3
Analysis of the oligomeric state of a protein by the combination of size-exclusion chromatography and static light scattering. The different oligomers of a small single-domain protein (7.0 kDa) covalently attached to a ligand peptide were separated by SEC. The purification was performed on a HiLoad Superdex 75 16/60 column (GE Healthcare) in 50 mM Tris–HCl pH 8.5 containing 200 mM NaCl. The absorbance at 280 nm (solid line) was measured in series with the static light-scattering signal (dots) using a Wyatt Technologies miniDAWN. The molecular weights of the two peaks in the elution profile were calculated to be 7.3 and 13.7 kDa, respectively, which correlates well with the monomeric and dimeric protein forms. |
![[Figure 3]](gx5084fig3.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
