Figure 5
Effectiveness of different screening blocks at producing crystals at the Oxford node. Each block contains 96 reagents and drops containing crystals were annotated manually. The number of plates set up using each block is given in parentheses. The blocks are based on conditions from the following commercial screens: Block 1, Hampton Crystal Screens I and II; block 2, Emerald Wizard Screens I and II; block 3, Hampton PEG/Ion, Grid Screens PEG 6000 and AS; block 4, Hampton Natrix Screen and Crystal Screen Cryo; block 5, Hampton PEG/LiCl, NaCl, MPD and Quik Screen Phosphate. Block M consists of Molecular Dimensions MemStart and MemSys screens and is focused on screening membrane proteins (Walter et al., 2003, 2005). |