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Figure 4
The overall efficiency, relative to the total number of targets selected, at various stages of the procedure from target selection to NMR structure. PCR, successful PCR amplification. Expressed, total expression >0.5 mg per millilitre of bacterial culture. Soluble, soluble expression >0.5 mg per millilitre of bacterial culture. HSQC, sufficient amount of protein was purified for 15 N HSQC analysis. For the indicated number of selected targets either one (grey bar) or multiple (black) constructs were evaluated.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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