NMR in the SPINE Structural Proteomics project

AB, E.; Atkinson, A.R.; Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Brunner, K.; Diercks, T.; Dötsch, V.; Engelke, F.; Folkers, G.E.; Griesinger, C.; Gronwald, W.; Günther, U.; Habeck, M.; de Jong, R.N.; Kalbitzer, H.R.; Kieffer, B.; Leeflang, B.R.; Loss, S.; Luchinat, C.; Marquardsen, T.; Moskau, D.; Neidig, K.-P.; Nilges, M.; Piccioli, M.; Pierattelli, R.; Rieping, W.; Schippmann, T.; Schwalbe, H.; Travé, G.; Trenner, J.; Wöhnert, J.; Zweckstetter, M.; Kaptein, R.

doi:10.1107/S0907444906032070

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 4
The overall efficiency, relative to the total number of targets selected, at various stages of the procedure from target selection to NMR structure. PCR, successful PCR amplification. Expressed, total expression >0.5 mg per millilitre of bacterial culture. Soluble, soluble expression >0.5 mg per millilitre of bacterial culture. HSQC, sufficient amount of protein was purified for ¹⁵ N HSQC analysis. For the indicated number of selected targets either one (grey bar) or multiple (black) constructs were evaluated.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 62| Part 10| September 2006| Pages 1150-1161

doi:10.1107/S0907444906032070

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