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Figure 1
Purification of two fragments (*) of the SARS S protein as Fc fusions for crystal trial. The proteins were recovered from the supernatant of Sf9 cells 9 d post-infection and the recombinant proteins were captured and concentrated by lectin (Lens culnaris) chromatography. The lectin eluates were further purified by protein A affinity chromatography. The final yield was ∼1 mg per litre of infected culture (109 cells). The proteins shown are S119–410-Fc (lane 1) and S119–713-Fc (lane 2).

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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