view article

Figure 4
Deglycosylation of the receptor tyrosine phosphatase RPTPμ expressed transiently in 293T and 293S/GnT1−/− cells. 5 mg of purified protein was treated with 250 U of endoglycosidase (EndoH) at pH 5.2 for 6 h at 310 K in each case. The samples were then analysed by SDS–PAGE under reducing conditions; the band marked with an asterisk is EndoH. Expression of RPTPμ in 293S/GnT1−/− cells leads to a larger fraction of the protein being `nicked'. In contrast to the partial EndoH-sensitivity of the 293T-derived material, the 293S/GnT1−/−-derived protein is completely EndoH-sensitive.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds