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Figure 2
Effect of the F602S mutation on GR expression. Comparison of the protein expression of wild-type GR (lane 1) and F602S GR (lane 2) in the presence of 10 µM dexamethasone. The proteins shown are the soluble fractions from the Ni2+ column. Lanes 3–5 show the purification of the F602S LBD (lane 3, sample after thrombin digestion; lane 4, Ni2+ column flowthrough of the thrombin-digested material; lane 5, final purified protein; Bledsoe et al., 2002BB2).

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 63| Part 1| December 2007| Pages 72-79
doi:10.1107/S0907444906047020
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