Figure 5
Effect of the C808S mutation and ligand type on MR expression. Although the C808S mutation is equivalent to the GR F602S mutation, it has a more pronounced effect on GST-MR LBD expression. This mutation produced increased expression of MR in the presence of a variety of ligands (L1, ligand 1; L2, ligand 2; L3, ligand 3). High levels of protein expression still require the presence of ligand during cell growth. |