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Figure 2
A comparison of data from SEC (a) and AUC (b) on the same protein. SEC provides a weight that is close to that expected for a dimer, whereas AUC shows a peak for the weight of a tetramer. It is likely that the result using SEC indicates that the protein (which is membrane-associated) interacts with the column matrix, leading to retardation and an erroneously low estimation of weight.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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