Figure 1
(a) Schematic diagram of an isothermal titration calorimetry instrument, consisting of a sample cell that contains one binding component, an automated syringe that contains the other binding partner and a reference cell. (b) Differences between the sample-cell and reference-cell temperatures induced by binding are initially translated to the power needed to bring the two samples back to the same temperature, before conversion to a binding enthalpy in molar terms. |