 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 1]](hm5049fig1mag.jpg)
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Figure 1
Schematic depictions of the global hairpin-ribozyme fold and the RNA sequences used in this investigation. (a) Ribbon diagram of the four-way helical junction (4WJ) hairpin ribozyme (PDB code 1m5k ). The junction region, which is not present in minimal constructs, is shown in salmon. RNA and protein residues included for the purposes of forming the U1A crystallization platform are colored gray. Other strands are colored as described for (b). (b) Secondary structure of the minimal hinged hairpin ribozyme adapted from RNAview (Yang et al., 2003  ). The substrate strand is depicted in green, the S-turn strand in red and the 29-mer strand in blue. The linker position is colored orange. The helix–loop–helix domains are labeled loop A and loop B; helices are labeled H1–H4. The inset displays two alternate synthetic linkages: C3L and S9L. The site of enzymatic cleavage is highlighted by a star. The A−1 residue is a 2′-deoxy A in the C3L structure and a 2′-deoxy-2′-amino U in the S9L structure. Conserved residues are boxed; blue backgrounds indicate residues of the ribose zipper, yellow boxes indicate E-loop residues and gray circled residues belong to the S-turn. Hydrogen-bond pairings: open-square, Hoogsteen; open triangle, trans-sugar; open circle, Watson–Crick face; closed circle, wobble pair. Double and single lines indicate Watson–Crick pairs; black dashed lines indicate single hydrogen bonds. (c) Ribbon diagram of the minimal hinged hairpin ribozyme solved in this investigation. |
![[Figure 1]](hm5049fig1.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
