view article
Figure 3
Kinetic analysis of hairpin-ribozyme constructs used in this study. Substrate-cleavage reactions were carried out under single-turnover conditions. For each construct (circles, S9-linked; diamonds, C3-linked; triangles, junctionless, JL) time courses were fitted to a double-exponential kinetic equation (see §[link]2.4). Inset: representative gel images of the cleavage time course for the S9 and JL constructs; similar results were observed for the C3 construct (data not shown). Bands represent 5′-Cy5-labeled 13-mer substrate and 8-mer product strands.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 63| Part 7| June 2007| Pages 812-825
doi:10.1107/S090744490702464X
Follow Acta Cryst. D
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS